| 出品公司: | Promega |
|---|---|
| 载体名称: | pGEM-T Easy |
| 质粒类型: | 细菌表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 3015 bp |
| 5' 测序引物及序列: | T7，SP6，M13Fwd |
| 3' 测序引物及序列: | M13Rev |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | pGEM-T和pGEM-T Easy之间的唯一区别在于多克隆位点（MCS）。pGEM-T Easy Vector的MCS包含插入片段两侧的序列，这些序列可被限制性酶Not I和EcoR I识别。这允许使用这些酶中的任一种通过一次限制性消化将插入序列DNA去除。用于PCR产物TA克隆的亲本载体。 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | 非病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGEM-T Easy 3015 bp ds-DNA circular SYN 25-NOV-2013
DEFINITION Parental vector for TA cloning of PCR products. The insertion site
is flanked by BstZI, EcoRI, and NotI sites.
ACCESSION .
VERSION .
KEYWORDS pGEM-T Easy
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3015)
AUTHORS Promega
TITLE Direct Submission
JOURNAL Exported Saturday, August 1, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
COMMENT Linearize at EcoRV to create a TA cloning vector.
FEATURES Location/Qualifiers
source 1..3015
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
misc_feature 10..128
/note="MCS"
/note="multiple cloning site"
promoter complement(140..158)
/note="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(176..192)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 200..216
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(224..254)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
rep_origin complement(578..1166)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1337..2197)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2198..2302)
/gene="bla"
/note="AmpR promoter"
rep_origin complement(2380..2835)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS complement(2812..180)
/codon_start=1
/gene="lacZ (fragment)"
/product="LacZ-alpha fragment of beta-galactosidase"
/note="lacZ-alpha"
/translation="MTMITPSYLGDTIEYSSYASNALGALPYGRPAGGREFTSDIEFPR
PPWRPGACDVGPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA
RTDRPSQQLRSLNGEWTRPVAAH"
primer_bind 2976..2992
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 2999..2
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
61 atcactagtg aattcgcggc cgcctgcagg tcgaccatat gggagagctc ccaacgcgtt
121 ggatgcatag cttgagtatt ctatagtgtc acctaaatag cttggcgtaa tcatggtcat
181 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
241 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
301 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
361 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
421 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
481 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
541 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
601 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
661 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
721 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
781 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
841 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
901 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
961 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
1021 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
1081 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
1141 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
1201 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
1261 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
1321 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
1381 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
1441 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
1501 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
1561 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
1621 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
1681 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
1741 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
1801 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
1861 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
1921 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
1981 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
2041 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
2101 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
2161 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
2221 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
2281 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgat gcggtgtgaa
2341 ataccgcaca gatgcgtaag gagaaaatac cgcatcagga aattgtaagc gttaatattt
2401 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
2461 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
2521 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
2581 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
2641 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
2701 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
2761 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
2821 cgctacaggg cgcgtccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
2881 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
2941 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattgta
3001 atacgactca ctata
//
